Volume 13, Issue 2 (Summer & Autumn 2016)                   ASJ 2016, 13(2): 105-116 | Back to browse issues page

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Samanipour R, Karbasi S, Hashemibeni B. Comparing Behavior of Chondrocyte Cells on Different Polyhydroxybutyrate Scaffold Structure for Cartilage Tissue Engineering. ASJ 2016; 13 (2) :105-116
URL: http://anatomyjournal.ir/article-1-174-en.html
Comparing Behavior of Chondrocyte Cells on Different Polyhydroxybutyrate Scaffold Structure for Cartilage Tissue Engineering
Reza Samanipour 1, Saeed Karbasi2 , Batool Hashemibeni3
1- Department of Nuclear Engineering, Faculty of Nuclear Engineering and Basic Sciences, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
2- Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract:   (7574 Views)

Introduction: As the ability to repair cartilage tissue in body is limited, finding a suitable method for cartilage regeneration has gained the attention of many scholars. For this purpose, scaffold structure and morphology, along with cell culture on it, can be a novel method to treat cartilage injuries, osteoarthritis.
Methods: In this study, polyhydroxybutyrate (PHB) is selected as the scaffold. Firstly, PHB (6% w/v) solution was prepared using chloroform solvent by employing solvent and electrospinning methods. With regard to phase studies, morphology, and specifying agent groups, we used specific characterization devices such as Fourier transform infrared spectroscopy (FTIR). To
compare the behaviour of cellular scaffolds, they were divided into 2 groups of scaffolds, and the chondrocyte cells were cultured. To perform phase studies, analysis of MTT and trypan blue were carried out for measuring the viability and attachment on the surface of the scaffold, and the specification of scanning electron microscope (SEM) was employed to determine the morphology of the cells.
Results: Through performing MTT test on the first, third and seventh days, it was found that these types of scaffolds are significantly different from those in the control group (P<0.05). Scanning electron microscope (SEM) indicates good attachment of chondrocytes on all scaffolds. Results obtained from trypan blue exclusion test also indicated an increase in cell attachment on scaffolds.
Conclusion: Comparing cell behavior on two scaffolds indicates that cell attachment, cell growth and proliferation, and cell migration on the electrospun scaffold is better than the scaffold provided by using solvent casting approach.

Keywords: Cartilage tissue engineering, Polyhydroxybutyrate, Scaffolding, Casting solvent, Electro spinning
Full-Text [PDF 726 kb]   (3628 Downloads)    
Type of Study: Review |
Received: 2016/01/1 | Accepted: 2016/03/31 | Published: 2016/07/1
References
1. Hunter W. Of the structure and disease of articulating cartilages 1743. Clinical Orthopaedics & Related Research. 1995; 317:3-6. PMID: 7671493 [PMID]
2. Tuli R, Li WJ, Tuan RS. Current state of cartilage tissue engineering. Arthritis Research & Therapy. 2003; 5(5):235–38. doi: 10.1186/ar991 [DOI:10.1186/ar991]
3. Portocarrero G., Collins G., Livingston Arinzeh T. Challenges in cartilage tissue engineering. Journal of Tissue Science & Engineering. 2013; 4:e120. doi: 10.4172/2157-7552.1000e120 [DOI:10.4172/2157-7552.1000e120]
4. Lanza R, Langer R, Vacanti JP. Principles of tissue engineering. New York: Academic Press; 2011.
5. Smith IO, Liu Xh, Smith LA, Ma PX. Nanostructured polymer scaffolds for tissue engineering and regenerative medicine. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 2009; 1(2):226-36. doi: 10.1002/wnan.26 [DOI:10.1002/wnan.26]
6. O'Brien FJ. Biomaterials & scaffolds for tissue engineering. Mater Today. 2011; 14(3):88-95. doi: 10.1016/S1369-7021(11)70058-X [DOI:10.1016/S1369-7021(11)70058-X]
7. Gardian C, Ferroni L, Favero L, Stellini E, Stomaci D, Sivolella S, et al. Nanostructured biomaterials for tissue engineered bone tissue restoration. International Journal of Molecular Sciences. 2012; 13(1):737-57. doi: 10.3390/ijms13010737 [DOI:10.3390/ijms13010737]
8. Tsuji H, Suzuyoshi K. Environmental degradation of biodegradable polyesters 1. Poly (ε-caprolactone), poly [(R)-3-hydroxybutyrate], and poly (L-lactide) films in controlled static seawater. Polymer Degradation & Stability. 2002; 75(2):347-55. doi: 10.1016/s0141-3910(01)00240-3 [DOI:10.1016/S0141-3910(01)00240-3]
9. Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 2005; 26(33):6565-578. doi: 10.1016/j.biomaterials.2005.04.036 [DOI:10.1016/j.biomaterials.2005.04.036]
10. Williams SF, Martin DP, Horowitz DM, Peoples OP. PHA applications: addressing the price performance issue: I. Tissue engineering. International Journal of Biological Macromolecules. 1999; 25(1):111-21. doi: 10.1016/s0141-8130(99)00022-7 [DOI:10.1016/S0141-8130(99)00022-7]
11. Li WJ, Tuli R, Okafor C, Derfoul A, Danielson KG, Hall DJ, et al. A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. Biomaterials. 2005; 26(6):599-609. doi: 10.1016/j.biomaterials.2004.03.005 [DOI:10.1016/j.biomaterials.2004.03.005]
12. Thorvaldsson A, Stenhamre H, Gatenholm P, Walkenström P. Electrospinning of highly porous scaffolds for cartilage regeneration. Biomacromolecules. 2008; 9(3):1044-49. doi: 10.1021/bm701225a [DOI:10.1021/bm701225a]
13. Zheng R, Duan H, Xue J, Liu Y, Feng B, Zhao S, et al. The influence of Gelatin/PCL ratio and 3-D construct shape of electrospun membranes on cartilage regeneration. Biomaterials. 2014; 35(1):152-64. doi: 10.1016/j.biomaterials.2013.09.082 [DOI:10.1016/j.biomaterials.2013.09.082]
14. Sombatmankhong K, Suwantong O, Waleetorncheepsawat S, Supaphol P. Electrospun fiber mats of poly (3-hydroxybutyrate), poly (3-hydroxybutyrate-co-3-hydroxyvalerate), and their blends. Journal of Polymer Science Part B: Polymer Physics. 2006; 44(19):2923-933. doi: 10.1002/polb.20915 [DOI:10.1002/polb.20915]
15. Choi YS, Cha SM, Lee YY, Kwon SW, Park CJ, Kim M. Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse. Biochemical & Biophysical Research Communications. 2006; 345(2):631-37. doi: 10.1016/j.bbrc.2006.04.128 [DOI:10.1016/j.bbrc.2006.04.128]
16. Blitterswijk CA, Moroni L, Rouwkema J, Siddappa R, Sohier J. Tissue engineering–an introduction. New York: Academic Press; 2008. [DOI:10.1016/B978-0-12-370869-4.00024-0]
17. Haji Ali H. [Synthesis and evaluation of mechanical properties, degradation, and bioactivity of composite scaffold of bioactive glass/polyhydroxybutyrate particles for bone tissue engineering (Persian)] [MSc. thesis]. Tehran: Iran University of Science and Technology; 2011.
18. Heydar Khan Tehrani A. [Manufacturing nanocomposite hydroxyapatite-polyhydroxybutyrate (nHA-PHB) scaffold using electrospinning method for tissue engineering (Persian)] [MSc. thesis]. Isfahan: Isfahan University of Technology; 2010.
19. Subia B, Kundu J, Kundu S. Biomaterial scaffold fabrication techniques for potential tissue engineering applications. In: Eberli D, editor. Tissue Engineering. New York: Intech Open Access Publisher; 2010, p. 142-57. [DOI:10.5772/8581]
20. Mandl EW, van der Veen SW, Verhaar JA, van Osch GJ. Serum-free medium supplemented with high-concentration FGF2 for cell expansion culture of human ear chondrocytes promotes redifferentiation capacity. Tissue Engineering. 2002; 8(4):573-80. doi: 10.1089/107632702760240490 [DOI:10.1089/107632702760240490]
21. Gan L, Kandel RA. In vitro cartilage tissue formation by co-culture of primary and passaged chondrocytes. Tissue Engineering. 2007; 13(4):831-42. doi: 10.1089/ten.2006.0231 [DOI:10.1089/ten.2006.0231]
22. Panossian A, Ashiku S, Kirchhoff CH, Randolph MA, Yaremchuk MJ. Effects of cell concentration and growth period on articular and ear chondrocyte transplants for tissue engineering. Plastic & Reconstructive Surgery. 2001; 108(2):392-402. doi: 10.1097/00006534-200108000-00018 [DOI:10.1097/00006534-200108000-00018]
23. Naumann A, Dennis JE, Aigner J, Coticchia J, Arnold J, Berghaus A, et al. Tissue engineering of autologous cartilage grafts in three-dimensional in vitro macroaggregate culture system. Tissue Engineering. 2004; 10(11-12):1695-706. doi: 10.1089/ten.2004.10.1695 [DOI:10.1089/ten.2004.10.1695]
24. Aigner J, Tegeler J, Hutzler P, Campoccia D, Pavesio A, Hammer C, et al. Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. Journal of Biomedical Materials Research. 1998; 42(2):172-81. PMID: 9773813 https://doi.org/10.1002/(SICI)1097-4636(199811)42:2<172::AID-JBM2>3.0.CO;2-M [DOI:10.1002/(SICI)1097-4636(199811)42:23.0.CO;2-M]
25. Isogai N, Kusuhara H, Ikada Y, Ohtani H, Jacquet R, Hillyer J, et al. Comparison of different chondrocytes for use in tissue engineering of cartilage model structures. Tissue Engineering. 2006; 12(4):691-703. doi: 10.1089/ten.2006.12.691 [DOI:10.1089/ten.2006.12.691]
26. Hidaka C, Cheng C, Alexandre D, Bhargava M, Torzilli PA. Maturational differences in superficial and deep zone articular chondrocytes. Cell Tissue Research. 2006; 323(1):127-35. doi: 10.1007/s00441-005-0050-y [DOI:10.1007/s00441-005-0050-y]
27. Lambert JB, Shurvell HF. Introduction & experimental methods. In: Betsy A, editor. Organic Structural Spectroscopy. New Jersey: Prentice–Hall; 1998.
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